Provide support services to satisfy the overall operational objectives of the National Institute on Aging. The primary objective is to provide services and deliverables through performance of support services.
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Program and use computers to store, process, and analyze data - 1
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Interpret research or operational data - 2
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Record research or operational data - 3
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Collect and analyze biological data about relationships among and between organisms and their environment - 4
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Monitor and observe experiments, recording production and test data for evaluation by research personnel - 5
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Provides technical experience needed to assist in studying the basic principles of plant and animal life
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Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
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Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species
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Assists with performing basic laboratory experiments and procedure
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Analyze experimental data and interpret results to write reports and summaries of findings
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Cultivate, maintain, expand and freeze ES and iPS cell lines from mouse and human
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Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry
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Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies
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Generate primary cultures of cells for the generation of new iPS cell lines
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Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits)
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Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based technique)
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Perform primary cell cultures of mitotically inactivated feeder layers
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Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis
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Investigate state of pluripotency of ES and new iPS cell lines
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Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR
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Design primers as needed for cell characterization
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Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use, record or report
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Prepares, organizes and maintains various lab samples, supplies, and equipment
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Maintain laboratory equipment
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Analyze laboratory findings
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Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation
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Prepare data, results, reports as well as Excel spreadsheets and PowerPoint slides, for oral presentation and/or publication in journals; write and edit scientific papers
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Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s)
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Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies, instruments and equipment, and submit to primary investigator and/or lab manager for approval
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Learns new laboratory techniques and procedures
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Cultivate, maintain, expand and freeze ES and iPS cell lines from mouse and human
-
Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry
-
Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies
-
Generate primary cultures of cells for the generation of new iPS cell lines
-
Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits)
-
Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based techniques
-
Perform primary cell cultures of mitotically inactivated feeder layers
-
Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis
-
Investigate state of pluripotency of ES and new iPS cell lines
-
Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR
-
Design primers as needed for cell characterization
-
Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use, record or report
-
Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation
-
Prepare data, results, reports as well as Excel spreadsheets and PowerPoint slides, for oral presentation and/or publication in journals; write and edit scientific papers
-
Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s)
-
Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies, instruments and equipment, and submit to primary investigator and/or lab manager for approval
